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Title: Visualization of Aspalathin in Rooibos (Aspalathus linearis) plant and herbal tea extracts using thin-layer chromatography
Authors: Stander, Emily Amor 
Williams, Wesley 
Rautenbach, Fanie 
Le Roes-Hill, Marilize 
Mgwatyu, Yamkela 
Marnewick, Jeanine L 
Hesse, Uljana 
Keywords: Rooibos;Aspalathus linearis;aspalathin;thin-layer chromatography;p-anisaldehyde;herbal tea;authentication;marker compound
Issue Date: 2019
Publisher: MDPI
Source: Stander, E. A., Williams, W., Rautenbach, F. et al. 2019. Visualization of Aspalathin in Rooibos (Aspalathus linearis) plant and herbal tea extracts using thin-layer chromatography. Molecules, 24: 938. 11pages. []
Journal: Molecules 
Abstract: Aspalathin, the main polyphenol of rooibos (Aspalathus linearis), is associated with diverse health promoting properties of the tea. During fermentation, aspalathin is oxidized and concentrations are significantly reduced. Standardized methods for quality control of rooibos products do not investigate aspalathin, since current techniques of aspalathin detection require expensive equipment and expertise. Here, we describe a simple and fast thin-layer chromatography (TLC) method that can reproducibly visualize aspalathin in rooibos herbal tea and plant extracts at a limit of detection (LOD) equal to 178.7 ng and a limit of quantification (LOQ) equal to 541.6 ng. Aspalathin is a rare compound, so far only found in A. linearis and its (rare) sister species A. pendula. Therefore, aspalathin could serve as a marker compound for authentication and quality control of rooibos products, and the described TLC method represents a cost-effective approach for high-throughput screening of plant and herbal tea extracts.
ISSN: 1420-3049
Appears in Collections:Appsc - Journal Articles (DHET subsidised)

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