Please use this identifier to cite or link to this item: http://hdl.handle.net/11189/6823
Title: Liquid chromatographic determination of the sphinganine/sphingosine ratio in serum
Authors: Shephard, Gordon Seymour 
van der Westhuizen, L 
Keywords: Sphinganine;Sphingosine;Fumonisin;Mycotoxin
Issue Date: 1998
Publisher: Elsevier
Source: Shephard GS, van der Westhuizen L. 1998. Liquid chromatographic determination of the sphinganine/sphingosine ratio in serum. Journal of Chromatography B: Biomedical Sciences and Applications, 710(1-2):219-222
Journal: Journal of Chromatography B: Biomedical Sciences and Applications, 710(1-2):219-222 
Abstract: The fumonisin mycotoxins, which are worldwide contaminants of corn, inhibit de novo sphingolipid biosynthesis leading to elevation in the ratio of the sphingoid bases, sphinganine and sphingosine, in the serum of animals exposed to fumonisins. A new HPLC method for the determination of the ratio of these bases in serum has been developed involving lipid extraction, clean-up on a silica minicolumn and alkaline hydrolysis prior to precolumn o-phthaldialdehyde derivatisation and HPLC separation and quantification by fluorescence detection. Based on serum from both normal and fumonisin-exposed vervet monkeys, the method was shown to be reproducible (R.S.D.<10%).
Description: Article
URI: http://hdl.handle.net/11189/6823
DOI: https://doi.org/10.1016/S0378-4347(98)00108-X
Appears in Collections:HWSci - Journal Articles (DHET subsidised)

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