Please use this identifier to cite or link to this item: http://hdl.handle.net/11189/6823
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dc.contributor.authorShephard, Gordon Seymouren_US
dc.contributor.authorVan der Westhuizen, Lianaen_US
dc.date.accessioned2019-02-01T09:36:44Z-
dc.date.available2019-02-01T09:36:44Z-
dc.date.issued1998-
dc.identifier.citationShephard. G.S. & Van der Westhuizen, L. 1998. Liquid chromatographic determination of the sphinganine/sphingosine ratio in serum. Journal of Chromatography B: Biomedical Sciences and Applications, 710(1-2):219-222en_US
dc.identifier.urihttp://hdl.handle.net/11189/6823-
dc.descriptionArticleen_US
dc.description.abstractThe fumonisin mycotoxins, which are worldwide contaminants of corn, inhibit de novo sphingolipid biosynthesis leading to elevation in the ratio of the sphingoid bases, sphinganine and sphingosine, in the serum of animals exposed to fumonisins. A new HPLC method for the determination of the ratio of these bases in serum has been developed involving lipid extraction, clean-up on a silica minicolumn and alkaline hydrolysis prior to precolumn o-phthaldialdehyde derivatisation and HPLC separation and quantification by fluorescence detection. Based on serum from both normal and fumonisin-exposed vervet monkeys, the method was shown to be reproducible (R.S.D.<10%).en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJournal of Chromatography B: Biomedical Sciences and Applications, 710(1-2):219-222en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/za/-
dc.subjectSphinganineen_US
dc.subjectSphingosineen_US
dc.subjectFumonisinen_US
dc.subjectMycotoxinen_US
dc.titleLiquid chromatographic determination of the sphinganine/sphingosine ratio in serumen_US
dc.type.patentArticleen_US
dc.identifier.doihttps://doi.org/10.1016/S0378-4347(98)00108-X-
Appears in Collections:HWSci - Journal Articles (DHET subsidised)
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