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dc.contributor.authorBrittle, Wen_US
dc.contributor.authorMarais, B. Jen_US
dc.contributor.authorHesseling, A. Cen_US
dc.contributor.authorSchaaf, H. Sen_US
dc.contributor.authorKidd, Men_US
dc.contributor.authorWasserman, Een_US
dc.contributor.authorBotha, Ten_US
dc.identifier.citationBrittle, W., Marais, B. J., Hesseling, A. C. et al. 2009. Improvement in Mycobacterial yield and reduced time to detection in pediatric samples by use of a nutrient broth growth supplement. Journal of Clinical Microbiology, 47(5): 1287–1289. []en_US
dc.description.abstractThere is an urgent need to improve the methods used for the bacteriological diagnosis of childhood mycobacterial disease. This study compared the mycobacterial yields and the times to detection (in days) of mycobacteria in pediatric clinical specimens by using Mycobacterial Growth Indicator Tubes (MGITs) and solid Lo¨wenstein-Jensen (LJ) slants with and without a nutrient broth supplement. A total of 801 specimens from 493 patients were processed: 82.8% were gastric aspirate specimens, 15.6% were sputum specimens, and 1.6% were fine-needle-aspiration biopsy specimens. The mycobacterial yield obtained with MGITs (with and without nutrient broth) was 11.0%, and that obtained with LJ slants was 1.6% (P < 0.001). Of the 88 positive cultures, 62 were detected in MGITs and 73 were detected in MGITs supplemented with nutrient broth (P 0.11). The mean time to detection in MGITs (without nutrient broth) was 18.5 days, whereas it was 12.4 days in MGITs with nutrient broth (P < 0.001). Supplementation of standard MGITs improved the mycobacterial yield and significantly reduced the time to detection of mycobacteria in pediatric samples.en_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.ispartofJournal of Clinical Microbiologyen_US
dc.subjectMycobacterial yielden_US
dc.subjectPediatric samplesen_US
dc.subjectNutrient brothen_US
dc.subjectGrowth supplementen_US
dc.subjectchildhood mycobacterial diseaseen_US
dc.titleImprovement in Mycobacterial Yield and Reduced Time to Detection in Pediatric Samples by Use of a Nutrient Broth Growth Supplementen_US
Appears in Collections:HWSci - Journal Articles (DHET subsidised)
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