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Title: Multiple mycotoxin exposure determined by urinary biomarkers in rural subsistence farmers in the former Transkei, South Africa
Authors: Shephard, Gordon Seymour 
Burger, HM 
Gambacorta, L 
Gong, YY 
Krska, R 
Rheeder, JP 
Solfrizzo, M 
Srey, C 
Sulyok, M 
Visconti, A 
Warth, B 
van der Westhuizen, L 
Keywords: Biomarker;FB(1), FB(2), fumonisin B(1);Fumonisin;Joint FAO/WHO Expert Committee on Food Additives;Mycotoxin;Zearalenone;aflatoxin B(1), M(1);de-epoxydeoxynivalenol;deoxynivalenol, DON-GlcA, DON-glucuronide;immunoaffinity column;liquid chromatography tandem mass spectrometry;nivalenol;ochratoxin A;phosphate buffered saline;provisional maximum tolerable daily intake;ultra-performance liquid chromatograph(y);zearalenone-glucuronide;α-, β-ZOL;α-, β-zearalenol
Issue Date: 2013
Publisher: Elsevier
Source: Food Chemistry Toxicology. 2013 Dec; 62:217-25
Journal: Food and Chemistry Toxicology 
Abstract: Subsistence farmers are exposed to a range of mycotoxins. This study applied novel urinary multi-mycotoxin LC-MS/MS methods to determine multiple exposure biomarkers in the high oesophageal cancer region, Transkei, South Africa. Fifty-three female participants donated part of their maize-based evening meal and first void morning urine, which was analysed both with sample clean-up (single and multi-biomarker) and by a 'dilute-and-shoot' multi-biomarker method. Results were corrected for recovery with LOD for not detected. A single biomarker method detected fumonisin B1 (FB1) (87% incidence; mean±standard deviation 0.342±0.466 ng/mg creatinine) and deoxynivalenol (100%; mean 20.4±49.4 ng/mg creatinine) after hydrolysis with β-glucuronidase. The multi-biomarker 'dilute-and-shoot' method indicated deoxynivalenol-15-glucuronide was predominantly present. A multi-biomarker method with β-glucuronidase and immunoaffinity clean-up determined zearalenone (100%; 0.529±1.60 ng/mg creatinine), FB1 (96%; 1.52±2.17 ng/mg creatinine), α-zearalenol (92%; 0.614±1.91 ng/mg creatinine), deoxynivalenol (87%; 11.3±27.1 ng/mg creatinine), β-zearalenol (75%; 0.702±2.95 ng/mg creatinine) and ochratoxin A (98%; 0.041±0.086 ng/mg creatinine). These demonstrate the value of multi-biomarker methods in measuring exposures in populations exposed to multiple mycotoxins. This is the first finding of urinary deoxynivalenol, zearalenone, their conjugates, ochratoxin A and zearalenols in Transkei.
Description: Article
DOI: 10.1016/j.fct.2013.08.040
Appears in Collections:HWSci - Journal Articles (DHET subsidised)

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