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Title: Reversed-phase high-performance liquid chromatography of tenuazonic acid and related tetramic acids.
Authors: Shephard, Gordon Seymour 
Thiel, PG 
Sydenham, Eric W. 
Vleggaar, R 
Marasas, Walter F.O 
Keywords: Reversed-phase high-performance liquid chromatographic system;Fungal toxin;Tenuazonic acid;(5S,8S)-3-acetyl-5-sec.-butyltetramic acid
Issue Date: 1991
Publisher: Elsevier
Abstract: A reversed-phase high-performance liquid chromatographic system for the determination of the fungal toxin, tenuazonic acid, (5S,8S)-3-acetyl-5-sec.-butyltetramic acid, is described. The system utilizes a column packed with deactivated end-capped C18 silica with a high carbon load to overcome the problem of poor chromatographic performance of this beta-diketone on reversed-phase liquid chromatography which previously necessitated the use of anion-exchange, ligand-exchange or ion-pairing methods. The reversed-phase system allows the separation of tenuazonic acid from its (5R,8S)-diastereomer, allo-tenuazonic acid and was applied to the detection of tenuazonic acid in cultures of Alternaria alternata and Phoma sorghina. By means of diode-array ultraviolet detection, (5S)-3-acetyl-5-isopropyltetramic acid was observed in extracts of culture material. This metabolite was purified using the analytical reversed-phase system and was identified by 1H and 13C nuclear magnetic resonance spectroscopy.
URI: 10.1016/0378-4347(91)80124-U
ISSN: 0021-9673
Appears in Collections:HWSci - Journal Articles (DHET subsidised)

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