Please use this identifier to cite or link to this item: http://hdl.handle.net/11189/4704
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dc.contributor.authorVan Wyk, Jessy-
dc.contributor.authorWitthuhn, Corli R-
dc.contributor.authorBritz, Trevor J-
dc.date.accessioned2016-07-25T06:03:43Z-
dc.date.available2016-07-25T06:03:43Z-
dc.date.issued2011-
dc.identifier.urihttp://dx.doi.org/10.1016/j.idairyj.2010.09.004-
dc.identifier.urihttp://hdl.handle.net/11189/4704-
dc.description.abstractVitamin B12 and folate deficiencies in poor Southern African communities lead to mandatory fortification of basic foodstuffs. Since folate- and B12-enriched kefir offers a cheaper alternative, regimes to include Propionibacterium freudenreichii (PAB) strains into kefir grains (KG) were investigated: two levels of PAB cell concentrations; freeze-drying KG to preserve PAB activity; and repeated PAB culture additions. Elevated B12 and folate levels and PCR results confirmed inclusion of PAB in all KG. Repeated inoculations with freeze-dried cultures (PAB concentration 1 × 108 cfu mL−1) delivered the highest B12 and folate production rate and concentration after 3 d. The best treatment (freeze-dried inoculum (5 × 107 PAB cfu mL−1) reacted once, followed by freeze-drying) delivered 186% Recommended Dietary Allowance (RDA) (B12) and 19% RDA (folate) per 200 mL serving. Freeze-drying preserved PAB activity in KG.en_US
dc.description.sponsorshipNational Research Foundation and by Cape Peninsula University of Technology (CPUT)en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/za/en
dc.subjectVitamin B12en_US
dc.subjectPropionibacterium freudenreichii (PAB)en_US
dc.subjectKefir grains (KG)en_US
dc.subjectPoor Southern African communitiesen_US
dc.titleOptimisation of vitamin B12 and folate production by Propionibacterium freudenreichii strains in kefiren_US
dc.type.patentArticleen_US
Appears in Collections:Appsc - Journal Articles (DHET subsidised)
Prof. Jessy Van Wyk
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