Chryseobacterium carnipullorum sp. nov., isolated from raw chicken

Abstract

Three Gram-staining-negative, rod-shaped, non-spore-forming, non-motile, oxidase-positive, yellow pigmented and aerobic bacterial isolates designated 8_R23573, 9_R23581T and 10_R23577 were isolated from raw chicken at a broiler processing plant in Bloemfontein, South Africa. A polyphasic taxonomic approach was used to determine their exact taxonomic identities. Phylogenetic analysis of the 16S rRNA gene sequences showed that the three strains belonged to the genus Chryseobacterium, exhibiting the highest similarities to Chryseobacterium shigense DSM 17126T (98.6-99.2 %) and Chryseobacterium luteum DSM 18605T (98.3-98.7 %). The most abundant quinone was menaquinone MK-6 and the predominant cellular fatty acids were iso-15: 0, iso-17: 1 ω9c, iso-17: 0 3-OH and summed feature 3 (iso-16: 1 ω7c and/or iso-15: 0 2-OH), which supported the affiliation of the strains to the genus Chryseobacterium. The DNA G + C contents of the strains were 36.9, 36.7 and 36.6 mol% respectively. The DNA-DNA hybridization results gave relatedness values ranging from 78.8 to 87.2 % among the three strains and 23.4 to 56.1 % to the two nearest phylogenetic neighbours C. shigense DSM 17126T and C. luteum LMG 23785T. On the basis of the data from this polyphasic study, the three strains are concluded to represent a novel species of the genus Chryseobacterium for which the name Chryseobacterium carnipullorum sp. nov. is proposed. The type strain is 9_R23581T (=LMG 26732T=DSM 25581T).