http://hdl.handle.net/11189/3436 Sun, 16 Aug 2026 08:30:22 GMT 2026-08-16T08:30:22Z https://digitalknowledge.cput.ac.za:443/retrieve/20226/ http://hdl.handle.net/11189/3436 http://hdl.handle.net/11189/7158 Title: Performance of fusarium oxysporum EKT01/02 isolate in cyanide biodegradation system Authors: Akinpelu, Enoch Akinbiyi; Adetunji, Adewole Tomiwa; Ntwampe, Seteno Karabo Obed; Nchu, Felix; Mekuto, Lukhanyo Abstract: This study reports a cyanide resistant and/or tolerant fungus, isolated from the rhizosphere of Zea mays contaminated with cyanide-based pesticides. The isolate was characterised using molecular biology. The effect of free cyanide and heavy metals on the growth of isolate in a synthetic gold mine wastewater was examined. The molecular analyses identified the isolate as Fusarium oxysporum EKT01/02 (KU985430/KU985431). The isolate had a free cyanide degradation efficiency of 77.6%. The results indicated greater growth impairment in culture containing Arsenic (optical density 1.28 and 1.458) and cyanide (optical density 1.315 and 1.385). Higher growth was observed in all cultures supplemented with extracellular polymeric substance. This study showed that the isolate possesses wide substrate utilisation mechanism that could be deployed in environmental engineering applications. Mon, 01 Jan 2018 00:00:00 GMT http://hdl.handle.net/11189/7158 2018-01-01T00:00:00Z http://hdl.handle.net/11189/7086 Title: Process performance determination data in thiocyanate biodegradation systems : use of sulphate production Authors: Mekuto, Lukhanyo; Razanamahandry, Lovasoa C.; Ntwampe, Seteno Karabo Obed; Mudumbi, John Baptist Nzukizi; Muchatibaya, Gift Abstract: This data article presents the utilization of sulphates as an indirect technique for the assessment of microbial growth, activity and SCN- biodegradation efficiency since the TDO were observed to be unable to utilise the produced sulphates as a source of sulphur (Mekuto e al., 2017) [1] The TDO demonstrated complete SCN- biodegradation while also utilizing the produced ammonium. The production of SO42- from SCN- biodegradation had a good correlation in comparison to the traditional methods of assessing microbial growth and activity i.e. direct cell counts (DCC), heterotrophic counts (CFU) and fluorescein production from fluorescein diacetate (FDA). The concentration of the produced SO42- demonstrated a similar logarithmic trend with the FDA, DCC and CFU techniques, thus confirming that the production of SO42- from SCN- biodegradation systems can be utilised as an indirect technique for the assessment of microbial growth, activity and SCN- biodegradation performance. Wed, 17 Jan 2018 00:00:00 GMT http://hdl.handle.net/11189/7086 2018-01-17T00:00:00Z http://hdl.handle.net/11189/7080 Title: Performance of fusarium oxysporum EKT01/02 isolate in cyanide biodegradation system Authors: Akinpelu, Enoch Akinbiyi; Adetunji, Adewole Tomiwa; Ntwampe, Seteno Karabo Obed; Nchu, Felix; Mekuto, Lukhanyo Abstract: This study reports a cyanide resistant and/or tolerant fungus, isolated from the rhizosphere of Zea mays contaminated with cyanide-based pesticides. The isolate was characterised using molecular biology. The effect of free cyanide and heavy metals on the growth of isolate in a synthetic gold mine wastewater was examined. The molecular analyses identified the isolate as Fusarium oxysporum EKT01/02 (KU985430/KU985431). The isolate had a free cyanide degradation efficiency of 77.6%. The results indicated greater growth impairment in culture containing Arsenic (optical density 1.28 and 1.458) and cyanide (optical density 1.315 and 1.385). Higher growth was observed in all cultures supplemented with extracellular polymeric substance. This study showed that the isolate possesses wide substrate utilisation mechanism that could be deployed in environmental engineering applications. Thu, 01 Feb 2018 00:00:00 GMT http://hdl.handle.net/11189/7080 2018-02-01T00:00:00Z http://hdl.handle.net/11189/6305 Title: Bacterial profiling of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O’kiep) Authors: Erdogan, Innocentia Gugulethu; Moncho, T.; Mekuto, Lukhanyo; Ntwampe, Seteno Karabo Obed; Fosso-Kankeu, E.; Waanders, F Abstract: The data presented herein contains the bacterial community profile of a drinking water supply system (DWSS) supplying O’kiep, Namaqualand, South Africa. Representative samples from the source (Orange River) to the point of use (O’kiep), through a 150km DWSS used for drinking water distribution were analysed for bacterial content. PCR amplification of the 16S rRNA V1-V3 regions was undertaken using oligonucleotide primers 27F and 518R subsequent to DNA extraction. The PCR amplicons were processed using the illumina® reaction kits as per manufactures guidelines and sequenced using the illumina® MiSeq-2000, by means of MiSeq V3 kit. The data obtained was processed using a bioinformatics QIIME software with a compatible fast nucleic acid (fna) file. The raw sequences were deposited at the National Centre of Biotechnology (NCBI) and the Sequence Read Archive (SRA) database, obtaining accession numbers for each species identified. The data revealed the presence of pathogenic bacteria from source to the point- of -use such as, Pseudomonas sp., Bacillus sp., Mycobacterium, Cyanobacterium (blue-green algae), Actinobacterium. Sun, 01 Jan 2017 00:00:00 GMT http://hdl.handle.net/11189/6305 2017-01-01T00:00:00Z