Please use this identifier to cite or link to this item: http://hdl.handle.net/11189/7473
Title: Peripheral blood mononuclear cell membrane fluidity and disease outcome in patients with multiple sclerosis
Authors: Hon, Gloudina Mary 
Hassan, Mogamat Shafick 
Van Rensburg, Susan Janse 
Abel, Stefan 
Erasmus, Rajiv T 
Matsha, Tandi Edith 
Keywords: Multiple sclerosis;Fatty acids;Phospholipids;Membrane fluidity
Issue Date: 2012
Publisher: Springer
Source: Hon, G. M., Hassan, M. S., Van Rensburg, S. J. et al. 2012. Peripheral blood mononuclear cell membrane fluidity and disease outcome in patients with multiple sclerosis. Indian Journal of Hematology and Blood Transfusion, 28(1): 1-6. [http://doi.org/10.1007/s12288-011-0087-x]
Journal: Indian Journal of Hematology and Blood Transfusion 
Abstract: Abstract Immune cell membrane lipids are important determinants of membrane fluidity, eicosanoid production and phagocytosis and fatty acid metabolic abnormalities have been reported in immune cells from patients with multiple sclerosis. The aim of this study was to investigate the relationship between peripheral blood mononuclear cell membrane fluidity, permeability status, and disease out- come as measured by the Kurtzke expanded disability status scale. Phospholipids, fatty acids and cholesterol composition in peripheral blood mononuclear cells from 26 patients diagnosed with multiple sclerosis and 25 healthy control subjects were determined by colorimetric assay, gas chromatography and enzymatic assays, respectively. Membrane fluidity was calculated according to previously established formulae and correlated with C-reactive protein and the Kurtzke expanded disability status scale. There were no significant differences in membrane lipids in peripheral blood mononuclear cells from patients and controls. However, correlation studies showed lipid meta- bolic abnormalities, which were reflected in significant correlations between membrane fluidity as measured by both its fatty acid and phospholipid compositions, and the functional system scores. C-reactive protein showed posi- tive correlations with phosphatidylcholine, phosphatidyl- serine, phosphatidylinositol and total phospholipids in membranes from control subjects. Metabolic abnormalities, as well as correlations between membrane fluidity and the functional system scores, suggested the involvement of these immune cell membranes in the disease progression. Furthermore, the changed relationship between membrane phospholipids and C-reactive protein, which has been shown to correlate with infectious episodes and clinical relapse, could be an indication of immune cell dysfunction in patients with multiple sclerosis
URI: http://hdl.handle.net/11189/7473
ISSN: 0971-4502
0974-0449
DOI: http://doi.org/10.1007/s12288-011-0087-x
Appears in Collections:HWSci - Journal Articles (DHET subsidised)

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