Please use this identifier to cite or link to this item: http://hdl.handle.net/11189/7270
Title: Effects of various medium compositions and wounding treatments on in vitro growth and regeneration of bird of paradise(Strelitzia reginae)
Authors: North, Jade J. 
Ndakidemi, Patrick Alois 
Laubscher, Charles P 
Keywords: Ascorbic acid;activated charcoal;6-benzylaminopurine (BAP);callus formation;discoloration;1- naphthalene acetic acid (NAA)
Issue Date: 2012
Publisher: Academic Journals
Source: North, J.J., Ndakidemi, P.A. & Laubscher, C.P. 2012. Effects of various medium compositions and wounding treatments on in vitro growth and regeneration of bird of paradise(Strelitzia reginae). Scientific Research and Essays, 7(10): 1118-1133. [http://dx.doi.org/10.5897/SRE11.601]
Journal: Scientific Research and Essays 
Abstract: The aim of this study was to investigate the use of antioxidants, wounding treatments and hormone concentrations in efforts to overcome phenolic oxidation and stimulate axillary bud proliferation. Significant results were achieved with 1-naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP) concentrations on explants discoloration and callus formation. The antioxidant treatments, activated charcoal (AC) and ascorbic acid (AA) significantly affected explant discoloration, the induction of callus and the length of roots developed. Wounding treatments resulted in a reduction of plant height, an increase in both explants discoloration and callus formation. The most effective treatment in reducing explants discoloration at the media contact point was achieved in interactive effects of higher NAA and BAP concentrations (0.1 mg.l-1 NAA; 3 mg.l-1 BAP and 0.5 mg.l-1 NAA; 5 mg.l-1 BAP) without wounding. Interactions between antioxidants and wounding treatments resulted in the absolute absence of callus induction in all treatments involving ascorbic acid.
URI: http://hdl.handle.net/11189/7270
ISSN: 1992-2248
DOI: http://dx.doi.org/10.5897/SRE11.601
Appears in Collections:Appsc - Journal Articles (not DHET subsidised)

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