Please use this identifier to cite or link to this item: http://hdl.handle.net/11189/6825
Title: Effect of fumonisin B1 on protein and lipid synthesis in primary rat hepatocytes
Authors: Gelderblom, WCA 
Smuts, CM 
Abel, S 
Snyman, SD 
Cawood, ME 
van der Westhuizen, L 
Swanevelder, S 
Issue Date: 1996
Publisher: Elsevier
Source: Gelderblom WCA, Smuts CM, Abel S, Snyman SD, Cawood ME, van der Westhuizen L & Swanevelder S. 1996. Effect of fumonisin B1 on protein and lipid synthesis in primary rat hepatocytes. Food and Chemical Toxicology, 34(4):361-369
Journal: Food and Chemical Toxicology, 34(4):361-369 
Abstract: The effect of fumonisin B1, (FB1) on protein and lipid synthesis was evaluated in primary rat hepatocytes. FB1 did not affect incorporation of [3H]leucine into hepatocytes at either non-toxic (150 μm) or cytotoxic (500 μm) concentrations indicating that protein synthesis was not affected. However, FB1 significantly (P < 0.01 to P < 0.0001) inhibited incorporation of [14C]palmitic acid into hepatocyte cultures implying that lipid synthesis was altered. Incorporation of the radiolabel was significantly (P < 0.05 to P < 0.0001) lowered in triacylglycerol (TAG) and sphingomyelin fractions and increased in phosphatidylcholine (PC) and phosphatidylethanolamine (PEA) in both FB1 concentrations. The incorporation pattern of [14C]palmitic acid closely resembles the changes in phospholipid levels in the treated cells. The sphingolipid, sphinganine (Sa), was significantly (P < 0.0001) increased in treated cells but there was no significant difference between the toxic and non-toxic dose levels implying that the increased Sa level alone is not responsible for the in vitro toxicity. FBI significantly (P < 0.01 to P < 0.001) decreased the level of free cholesterol within the cell, resulting in an increased PC:cholesterol ratio suggesting a more rigid membrane structure. Subsequent studies on the fatty acid (FA) profiles in PC and the neutral lipid, TAG, indicated that FBI significantly (P < 0.05 to P < 0.0001) increased the levels of the polyunsaturated FAs C18:2n-6 and C20:4n-6 at both concentrations. The FB1-induced changes to cellular membranes, specifically those related to FA changes in the major membrane phospholipids, and the altered FA content of the hepatocytes are likely to be key events in explaining the cytotoxic effects and altered growth responses induced by fumonisins in primary hepatocytes.
Description: Article
URI: http://hdl.handle.net/11189/6825
DOI: https://doi.org/10.1016/0278-6915(96)00107-X
Appears in Collections:HWSci - Journal Articles (DHET subsidised)

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