Please use this identifier to cite or link to this item: http://hdl.handle.net/11189/6733
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dc.contributor.authorGelderblom, W.C.A.en_US
dc.contributor.authorSnyman, SDen_US
dc.contributor.authorVan der Westhuizen, Len_US
dc.contributor.authorMarasas, Walter F.Oen_US
dc.date.accessioned2019-01-21T10:56:27Z-
dc.date.available2019-01-21T10:56:27Z-
dc.date.issued1995-
dc.identifier.citationGelderblom WCA, Snyman SD, van der Westhuizen L & Marasas WFO. 1995. Mitoinhibitory effect of fumonisin B1 on rat hepatocytes in primary culture. Carcinogenesis Integrative Cancer Research:16(3):625-31en_US
dc.identifier.urihttp://hdl.handle.net/11189/6733-
dc.descriptionArticleen_US
dc.description.abstractThe inhibitory effect of fumonisin B1 (FB1) on epidermal growth factor (EGF)-induced DNA synthesis in primary rat hepatocytes was investigated by monitoring the incorporation of [3H]thymidine in the DNA. A pulse-labelling technique was adapted to determine the incorporation of the radioactivity in the DNA (S-phase) quantitatively. FB1 inhibits the EGF-induced DNA synthesis up to 90% when incorporated at concentrations of 150 to 300 microM for a period of 44 h. A continued presence of FB1 is required to exhibit this inhibition as (i) the subsequent removal of FB1 resulted in a reversal of the effect, (ii) a higher stimulatory response in EGF-treated hepatocytes was found when the exposure period of hepatocytes in FB1 was reduced, and (iii) pretreatment of hepatocytes with FB1 only slightly reduced (not significantly) DNA synthesis induced by EGF. Whilst the growth inhibitory effect of FB1 was not associated with a cytotoxic effect, binding studies using [125I]EGF indicated that the growth factor-receptor interaction was not altered. No relationship was found between the disruption of the sphingolipid biosynthesis by FB1 and (i) the mitoinhibitory effect on the EGF response and (ii) the cytotoxicity of FB1 in primary hepatocytes.en_US
dc.language.isoenen_US
dc.publisherOxford University Pressen_US
dc.relation.ispartofCarcinogenesis Integrative Cancer Research:16(3):625-31en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/za/-
dc.subjectgrowth factor receptorsen_US
dc.subjectdnaen_US
dc.subjecthepatocytesen_US
dc.subjectradioactivityen_US
dc.subjectchemical synthesisen_US
dc.subjectfumonisinsen_US
dc.subjectratsen_US
dc.subjectcytotoxicityen_US
dc.titleMitoinhibitory effect of fumonisin B1 on rat hepatocytes in primary culture.en_US
dc.type.patentArticleen_US
dc.identifier.pmid7697823-
Appears in Collections:HWSci - Journal Articles (DHET subsidised)
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