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Title: Assessment of Pathogen Reduction Potential of an Anaerobic Digester containing Winery Wastewater
Authors: Okudoh, Vincent Ifeanyi 
Okeleye, Benjamin I. 
Ngwe, Ronny. M 
Ntwampe, Seteno Karabo Obed 
Keywords: Anaerobic digestion;biogas;methanogens;pathogen reduction
Issue Date: 2018
Publisher: North-West University (NWU) and Eminent Association of Pioneers (EAP)
Series/Report no.: ;11
Conference: 10th Int'l Conference on Advances in Science, Engineering, Technology & Healthcare (ASETH-18) Nov. 19-20, 2018 Cape Town (South Africa) 
Abstract: In this study, assessment of the pathogenic bacteria reduction capacity of an anaerobic digester (AD) containing winery wastewater (WWW) was carried out. Samples were collected from a winery drainage system (influent) and a bioreactor after digestion (effluent) at the Agricultural Research Council (ARC), Stellenbosch, South Africa. Qualitative analysis was by means of pH, optical density (OD) and most probable number (MPN) techniques. The isolates were identified using biochemical and polymerase chain reaction (PCR) methods. Results showed an increase in pH from 6.71 to 6.97 with MPN index per 100 mL reduction of presumptive coliforms from >1,100 to 313.3 after digestion at 95% confidence interval (CI), respectively. A 3 log removal efficiency (99.9%) of Escherichia coli, Salmonella spp., Azomonas spp. and Aeromonas hydrophila was achieved after 30 days retention time in the bio-digester. The significant reduction of these surrogate pathogens during the process of biogas production is of importance to mitigate against environmental health concerns.
Description: Conference Proceeding
ISBN: 9788193836521
Appears in Collections:Appsc - Conference Proceedings

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