Please use this identifier to cite or link to this item: http://hdl.handle.net/11189/6504
Title: Small laccase-catalyzed synthesis of a caffeic acid dimer with high antioxidant capacity
Authors: Nemadziva, Blessing 
Le Roes-Hill, Marilize 
Koorbanally, Neil 
Kudangaa, Tukayi 
Keywords: Small laccase;Caffeic acid;Coupling reactions;Antioxidant activity of flavonoids;Physicochemical properties
Issue Date: 2018
Publisher: Process Biochemistry
Journal: Process Biochemistry 
Abstract: The increase in the demand for antioxidant compounds for application in industries such as food and pharmaceutical, has necessitated the need to investigate new production methods The application of laccase for antioxidant synthesis is gaining notable attention as a viable approach to address this need. In this study, a β-β caffeic acid dimer, phellinsin A, was synthesized using the small laccase, SLAC, a two-domain bacterial laccase native to Streptomyces coelicolor A3(2). Phellinsin A showed a 1.5-fold increase in 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity and a 1.8-fold improvement in trolox equivalence antioxidant capacity (TEAC) when compared to caffeic acid. Phellinsin A also showed improved solubility properties in aqueous media and remarkable stability at acidic pH (pH 2.2 and pH 5.5). This study demonstrates the potential of the bacterial laccase, SLAC, as a catalyst for the biotransformation of natural phenolics into value-added compounds.
URI: https://doi.org/10.1016/j.procbio.2018.03.009
http://hdl.handle.net/11189/6504
ISSN: 1873-3298
Appears in Collections:HWSci - Journal Articles (DHET subsidised)

Show full item record

Page view(s)

19
checked on Jul 16, 2019

Google ScholarTM

Check


This item is licensed under a Creative Commons License Creative Commons