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Title: Metabolic engineering of malolactic wine yeast
Authors: Husnik, John I 
Volschenk, Heinrich 
Bauer, Jurgen 
Colavizza, Didier 
Luo, Zongli 
Van Vuuren, Hennie JJ 
Keywords: Saccharomyces cerevisiae;Oenococcus oeni;Malolactic fermentation;Malate permease;Malolactic enzyme
Issue Date: 2006
Publisher: Elsevier
Source: Husnik, J.I., Volschenk, H., Bauer, J., Colavizza, D., Luo, Z. and van Vuuren, H.J. 2006. Metabolic engineering of malolactic wine yeast. Metabolic Engineering, 8(4): 315-323
Journal: Metabolic Engineering 
Abstract: Malolactic fermentation is essential for the deacidification of high acid grape must. We have constructed a genetically stable industrial strain of Saccharomyces cerevisiae by integrating a linear cassette containing the Schizosaccharomyces pombe malate permease gene (mae1) and the Oenococcus oeni malolactic gene (mleA) under control of the S. cerevisiae PGK1 promoter and terminator sequences into the URA3 locus of an industrial wine yeast. The malolactic yeast strain, ML01, fully decarboxylated 5.5 g/l of malate in Chardonnay grape must during the alcoholic fermentation. Analysis of the phenotype, genotype, transcriptome, and proteome revealed that the ML01 yeast is substantially equivalent to the parental industrial wine yeast. The ML01 yeast enjoys ‘Generally Regarded As Safe’ status from the FDA and is the first genetically enhanced yeast that has been commercialized. Its application will prevent the formation of noxious biogenic amines produced by lactic acid bacteria in wine.
ISSN: 1096-7176
Appears in Collections:Appsc - Journal Articles (DHET subsidised)

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