Please use this identifier to cite or link to this item: http://hdl.handle.net/11189/6305
Title: Bacterial profiling of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O’kiep)
Authors: Erdogan, Innocentia Gugulethu 
Moncho,T 
Mekuto, L 
Ntwampe, Seteno KO 
Fosso-Kankeu, E 
Waanders, F 
Keywords: Arid Region;Drinking water supply system (DWSS);O’kiep;16S rRNA gene
Issue Date: 2017
Source: Erdogan, I.G., Moncho, T., Ntwampe, S.K.O., Fosso-Kankeu, E., Waanders, F., 2017. Bacterial profiling of bacterial communities of a drinking water supply system (DWSS) in the arid Namaqualand region, South Africa: Source (lower Orange River) to point-of-use (O’kiep).
Abstract: The data presented herein contains the bacterial community profile of a drinking water supply system (DWSS) supplying O’kiep, Namaqualand, South Africa. Representative samples from the source (Orange River) to the point of use (O’kiep), through a 150km DWSS used for drinking water distribution were analysed for bacterial content. PCR amplification of the 16S rRNA V1-V3 regions was undertaken using oligonucleotide primers 27F and 518R subsequent to DNA extraction. The PCR amplicons were processed using the illumina® reaction kits as per manufactures guidelines and sequenced using the illumina® MiSeq-2000, by means of MiSeq V3 kit. The data obtained was processed using a bioinformatics QIIME software with a compatible fast nucleic acid (fna) file. The raw sequences were deposited at the National Centre of Biotechnology (NCBI) and the Sequence Read Archive (SRA) database, obtaining accession numbers for each species identified. The data revealed the presence of pathogenic bacteria from source to the point- of -use such as, Pseudomonas sp., Bacillus sp., Mycobacterium, Cyanobacterium (blue-green algae), Actinobacterium.
URI: http://hdl.handle.net/11189/6305
Appears in Collections:BioERG - Research Data

Show full item record

Page view(s)

53
checked on Mar 16, 2019

Download(s)

10
checked on Mar 16, 2019

Google ScholarTM

Check


This item is licensed under a Creative Commons License Creative Commons