Please use this identifier to cite or link to this item: http://hdl.handle.net/11189/6187
Title: Bioremediation of polycyclic aromatic hydrocarbon (PAH) compounds: (acenaphthene and fluorene) in water using indigenous bacterial species isolated from the Diep and Plankenburg rivers, Western Cape, South Africa
Authors: Alegbeleye, Oluwadara Oluwaseun 
Opeolu, Beatrice Olutoyin 
Jackson, Vanessa 
Keywords: Polycyclic aromatic hydrocarbons (PAHs);Bioremediation;Bioreactor;PAH degrading microorganisms
Issue Date: 2017
Publisher: Brazilian Journal of Microbiology
Journal: Brazilian Journal of Microbiology 
Abstract: This study was conducted to investigate the occurrence of PAH degrading microorganisms in two river systems in the Western Cape, South Africa and their ability to degrade two PAH compounds: acenaphthene and fluorene. A total of 19 bacterial isolates were obtained from the Diep and Plankenburg rivers among which four were identified as acenaphthene and fluorene degrading isolates. In simulated batch scale experiments, the optimum temperature for efficient degradation of both compounds was determined in a shaking incubator after 14 days, testing at 25 °C, 30 °C, 35 °C, 37 °C, 38 °C, 40 °C and 45 °C followed by experiments in a Stirred Tank Bioreactor using optimum temperature profiles from the batch experiment results. All experiments were run without the addition of supplements, bulking agents, biosurfactants or any other form of biostimulants. Results showed that Raoultella ornithinolytica, Serratia marcescens, Bacillus megaterium and Aeromonas hydrophila efficiently degraded both compounds at 37 °C, 37 °C, 30 °C and 35 °C respectively. The degradation of fluorene was more efficient and rapid compared to that of acenaphthene and degradation at Stirred Tank Bioreactor scale was more efficient for all treatments. Raoultella ornithinolytica, Serratia marcescens, Bacillus megaterium and Aeromonas hydrophila degraded a mean total of 98.60%, 95.70%, 90.20% and 99.90% acenaphthene, respectively and 99.90%, 97.90%, 98.40% and 99.50% fluorene, respectively. The PAH degrading microorganisms isolated during this study significantly reduced the concentrations of acenaphthene and fluorene and may be used on a larger, commercial scale to bioremediate PAH contaminated river systems.
URI: http://hdl.handle.net/11189/6187
ISSN: 1517-8382
DOI: http://dx.doi.org/10.1016/j.bjm.2016.07.027
Appears in Collections:Appsc - Journal Articles (DHET subsidised)

Show full item record

Page view(s)

37
checked on Jun 18, 2019

Google ScholarTM

Check

Altmetric


This item is licensed under a Creative Commons License Creative Commons