Please use this identifier to cite or link to this item: http://hdl.handle.net/11189/5978
Title: Rooibos (Aspalathus linearis), honeybush (Cyclopia intermedia) and cancer bush (Sutherlandia frutescens subsp. microphylla) protect against tobacco-specific mutagenesis in vitro
Authors: Gelderblom, Wentzel CA 
Joubert, E 
Gamieldien, K 
Sissing, L 
Malherbe, CJ 
Maritz, G 
Keywords: Antimutagenesis;Tobacco-specific mutagens;Herbal teas;Cancer bush
Issue Date: 2017
Publisher: Elsevier
Abstract: Antimutagenesis studies against the tobacco-specific mutagens 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and N-oxide, 4 (methyl-nitrosamino)-1-(3-pyridyl)-1-butanol (NNAL), were conducted using hot water aqueous extracts of rooibos (Aspalathus linearis), honeybush (Cyclopia intermedia), and cancer bush (Sutherlandia frutescens). Aqueous extracts of both “fermented” and “unfermented” (green) rooibos and honeybush were included, while extracts of green and black teas (Camellia sinensis) served as benchmarks. A polyphenol-enriched methanol extract of unfermented rooibos (RgM) was included to further elucidate the possible role of rooibos polyphenols. Studies were performed in the presence of the metabolic activation against Salmonella typhimurium tester strain TA1535, using the standard plate incorporation and micro-suspension, pre-incubation assays. The mutagenic effects of NNK against the strain TA1535 was best demonstrated using the standard plate incorporation assay, while a higher mutagenicity was demonstrated for NNAL using the micro-suspension, pre-incubation method. Black tea and RgM exhibited the highest protection against NNK-induced mutagenesis followed by the aqueous extracts of rooibos ≥ green tea ≥ honeybush ≥ cancer bush. Black tea, green tea, RgM and unfermented rooibos were the most effective against NNAL-induced mutagenesis, followed by fermented rooibos. The two honeybush extracts exhibited similar, but the weakest protective response. When considering the amount of total polyphenols (TPP) incorporated in the plate incorporation assay, cancer bush exhibited similar protection to that of fermented and unfermented honeybush against NNK mutagenesis. The involvement of specific polyphenol-cytochrome P450 (CYP450) interactions is likely to be involved in the protection against tobacco-related mutagenesis. Polyphenol constituents of rooibos, honeybush and cancer bush could play an important role in the protection against mutagenesis induced by the major tobacco-specific carcinogens.
URI: https://doi.org/10.1016/j.sajb.2016.06.004
http://hdl.handle.net/11189/5978
ISSN: 0254-6299
Appears in Collections:HWSci - Journal Articles (DHET subsidised)

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