Please use this identifier to cite or link to this item: http://hdl.handle.net/11189/5770
Title: Enhanced antimicrobial and anticancer activity of silver and gold nanoparticles synthesised using sargassum incisifolium aqueous extracts.
Authors: Mmola, Mokone 
Roes-Hill, Marilize. L 
Durrell, Kim 
Bolton, John. J 
Sibuyi, Nicole 
Meyer, Mervin. E 
Beukes, Denzil. R 
Antunes, Edith 
Keywords: Silver nanoparticles;Gold nanoparticles;Green synthesis;Fucoidans;Sargassum incisifolium;Antibacterial activity;Cytotoxicity;Total phenolic content
Issue Date: 2016
Publisher: PubMed
Abstract: A detailed, methodical approach was used to synthesise silver and gold nanoparticles using two differently prepared aqueous extracts of the brown algae Sargassum incisifolium. The efficiency of the extracts in producing nanoparticles were compared to commercially available brown algal fucoidans, a major constituent of brown algal aqueous extracts. The nanoparticles were characterised using TEM, XRD and UV/Vis spectroscopy and zeta potential measurements. The rate of nanoparticle formation was assessed using UV/Vis spectroscopy and related to the size, shape and morphology of the nanoparticles as revealed by TEM. The antioxidant, reducing power and total polyphenolic contents of the aqueous extracts and fucoidans were determined, revealing that the aqueous extracts with the highest contents produced smaller, spherical, more monodisperse nanoparticles at a faster rate. The nanoparticles were assessed against two gram-negative bacteria, two gram-positive bacteria and one yeast strain. In contrast to the literature, the silver nanoparticles produced using the aqueous extracts were particularly toxic to Gram-negative bacteria, while the gold nanoparticles lacked activity. The cytotoxic activity of the nanoparticles was also evaluated against cancerous (HT-29, MCF-7) and non-cancerous (MCF-12a) cell lines. The silver nanoparticles displayed selectivity, since the MCF-12a cell line was found to be resistant to the nanoparticles, while the cancerous HT-29 cell line was found to be sensitive (10% viability). The gold nanoparticles displayed negligible toxicity.
URI: http://dx.doi.org/10.3390/molecules21121633
http://hdl.handle.net/11189/5770
ISSN: 1420-3049
Appears in Collections:HWSci - Journal Articles (DHET subsidised)

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