Please use this identifier to cite or link to this item: http://hdl.handle.net/11189/5665
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dc.contributor.authorVan Zijl, Len_US
dc.contributor.authorBester, Andreen_US
dc.contributor.authorGevers, Wen_US
dc.contributor.authorBoyd, Cen_US
dc.date.accessioned2017-06-08T13:35:28Z-
dc.date.available2017-06-08T13:35:28Z-
dc.date.issued1983-
dc.identifier.citationFEBS Letters, vol. 159, issue 1-2 (1983) pp. 33-38en_US
dc.identifier.issn00145793-
dc.identifier.urihttp://dx.doi.org/10.1016/0014-5793(83)80411-6-
dc.identifier.urihttp://hdl.handle.net/11189/5665-
dc.description.abstractRibonucleotidyl transferases are a group of primer-dependent terminal transferases, of nuclear and cytoplasmic origin, that have been known to exist for many years [l-6]. The best known perhaps are the cytoplasmic tRNA nucleotidyl transferases, shown some time ago to be responsible for the addition of the 3 ’ -terminal trinucleotide CCA to tRNA [7,8]. Poly(A)polymerase activities have also been described and shown to play a role in the polyadenylation of newly synthesized premRNA [g-11]. We have been concerned with a terminal transferase activity in the ribosome fraction of maturing avian erythrocytes responsible for the synthesis of polymers of oligo(U) [12]. We have shown the product of this enzyme activity to be present in the cytoplasm of intact cells [ 131, but the enzyme is not related to cytoplasmic tRNA nucleotidyl transferase [14]. This lends considerable support to the idea that this terminal transferase is not an artefact of isolation or assay. What then is the function of this enzyme? We have previously provided correlative evidence [ 121 suggesting that such a ribosome-associated terminal transferase may play a role in the synthesis or control of synthesis of globin, during the development of the immature avian erythrocyte. In order to investigate a possible translational control function more directly we have continued these studies using developing embryonic chick skeletal muscle. The development of mononucleated myoblasts to form nonproliferating myotubes has been extensively studied in terms of the synthesis of musclespecific proteins and the translational regulation of this synthesis [ 15,161. It represents therefore a suitable system for the analysis of primerdependent terminal transferases and their possible role in regulating differential mRNA availability and translation. This paper reports the presence of cytoplasmic terminal transferases in developing chick embryo skeletal muscle and the changes in the activities of these polysome- and 80 S-associated terminal transferases during myoblast development in vivo.en_US
dc.language.isoenen_US
dc.publisherFederation of European Biochemical Societiesen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/za/-
dc.subjectRibonucleotidyl transferasesen_US
dc.subjectNuclear and cytoplasmic originen_US
dc.subjectChick embryoen_US
dc.subjectSkeletal muscle developmenten_US
dc.titleChanges in cytoplasmic terminal transferase activities during chick embryo skeletal muscle developmenten_US
dc.type.patentArticleen_US
Appears in Collections:Eng - Journal articles (DHET subsidised)
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