Please use this identifier to cite or link to this item: http://hdl.handle.net/11189/5358
Title: Comparison of the growth kinetics and proteolytic activities of chryseobacterium species and pseudomonas fluorescens
Authors: Bekker, A 
Steyn, L 
Charimba, George 
Jooste, P 
Hugo, C 
Keywords: pseudomonas fluorescens;food spoilage;chryseobacterium;spoilage;milk;temperature profiles;proteolysis;UHT treatment;absorbance
Issue Date: 2015
Publisher: NRC Research Press (Canadian Science Publishing)
Source: Bekker, A., Steyn, L., Charimba, G., Jooste, P. and Hugo, C. 2015. Comparison of the growth kinetics and proteolytic activities of chryseobacterium species and pseudomonas fluorescens. Canadian Journal of Microbiology, 61(12): 977-982.
Abstract: The effect of temperature on the growth kinetics and proteolytic activity of Chryseobacterium joostei and Chryseobacterium bovis was determined during this study. The results were compared with the activities of Pseudomonas fluorescens, which is regarded to be a major food spoilage psychrotolerant microorganism. For the growth studies, cultures were incubated in nutrient broth in a temperature gradient incubator (from 9 to 50 °C) and separately at 4 °C, and the optical density was measured at different time intervals. Growth temperature profiles for each organism were constructed. For determination of proteolytic activity, the cultures were incubated in fat-free ultra-high temperature processed milk in the temperature gradient incubator for 72 h (temperature range as above). Cell-free extracts were used to determine the proteolytic activity using the azocasein method. Results of the growth studies showed that C.joostei had the ability to grow over a wider temperature range than C.bovis and P.fluorescens without being affected by changes in the temperature. For the proteolytic activity, C. joostei had significantly (p < 0.001) higher activity per milligram of protein at 15.5 °C, followed by C.bovis and P.fluorescens. The results showed that C.joostei potentially has an even greater spoilage capacity in milk on the basis of growth rate and proteolytic activity than did P.fluorescens.
URI: http://dx.doi.org/10.1139/cjm-2015-0236
http://hdl.handle.net/11189/5358
ISSN: 0008-4166
1480-3275
Appears in Collections:Dr. George Charimba

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