Please use this identifier to cite or link to this item: http://hdl.handle.net/11189/5291
Title: Optimization of biosurfactant production by Bacillus licheniformis STK 01 grown exclusively on beta vulgaris waste using response surface methodology
Authors: Amodu, Olusola Solomon 
Ntwampe, Seteno KO 
Ojumu, Tunde 
Keywords: Agricultural wastes;Biosurfactants;Emulsification;Surface tension;Central composite design
Issue Date: 2014
Publisher: BioResources
Source: Amodu, O.S., Ntwampe, S.K. and Ojumu, T.V., 2014. Optimization of biosurfactant production by Bacillus licheniformis STK 01 grown exclusively on Beta vulgaris waste using response surface methodology. BioResources, 9(3),5045-5065.
Abstract: This work reports on the exploitation of Beta vulgaris for biosurfactant production by Bacillus licheniformis STK 01 and its optimization using statistical modeling of response surface methodology (RSM). Three variables were investigated: agro-waste concentration, pH, and temperature. The response and contour plots of the RSM showed perfect interaction among the variables, with the highest surface tension reduction of the culture medium to 30 mN/m observed at 42 °C, a pH of 8, and a substrate concentration of 4% (w/v). The biosurfactant produced demonstrated a high tendency for hydrocarbon emulsification. Furthermore, by numerical optimization techniques, the optimum conditions were found to be as follows: a pH of 6.72, an agro-waste concentration of 4% (w/v), and a temperature of 44.5 °C. The experiment conducted to validate the optimum conditions obtained showed a biosurfactant with remarkable surface activity, lowering the surface tension of the broth to 30 mN/m, when the organism was grown on B. vulgaris, and to 23.5 mN/m, when grown in glucose medium – the later representing one of the highest surface tension reductions ever reported for a biosurfactant. This study revealed, among others, that the exclusive utilization of cheap solid agro-waste without supplementation with a refined nutrient source is feasible and could ensure the economic sustainability of biosurfactant production.
URI: http://hdl.handle.net/11189/5291
Appears in Collections:Prof. Seteno Karabo Ntwampe

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