Please use this identifier to cite or link to this item: http://hdl.handle.net/11189/5076
Title: In vitro regeneration of recalcitrant embryonic axes: Effects on the biomass characteristics of resulting plants
Authors: Cheruiyot, C 
Kioko, JI 
Berjak, P 
Wesley-Smith, J 
Keywords: Recalcitrant embryonic axes;Biomass characteristics of plants;Recalcitrant seeds;Cryopreservation of zygotic germplasm
Issue Date: 2007
Publisher: Elsevier
Abstract: In vitro regeneration of the embryonic axes of recalcitrant seeds is a necessary step in practices such as cryopreservation of zygotic germplasm, micropropagation, and the production of disease-free plants. As recalcitrant seeds usually harbour surface and tissue-borne microflora, axes used for in vitro culture are invariably subjected to decontamination treatments such as surface-sterilisation with antiseptic substances, treatment with surface- and systemic fungicides and antibiotics, and culturing on variously-modified media formulations. The effects of these treatments are usually evaluated only on the basis of the level of decontamination achieved, with little information available on how these decontamination treatments affect the establishment and/or biomass characteristics of the plants subsequently produced. This study investigated the effects of different in vitro culture media, surface-steriliants, phenolic-controlling compounds, systemic fungicides and antibiotics on biomass accumulation and partitioning of seedlings obtained from embryonic axes of Syzygium cordatum, a recalcitrant-seeded multipurpose tree species indigenous to eastern and southern Africa. Embryonic axes were found to be infected with tissue-borne fungi at harvest, and any development after in vitro culture depended on decontamination.
URI: http://dx.doi.org/10.1016/j.sajb.2007.04.006
http://hdl.handle.net/11189/5076
Appears in Collections:Appsc - Conference Papers

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