Please use this identifier to cite or link to this item: http://hdl.handle.net/11189/4759
Title: Effects of various media compositions on the in- vitro germination and discoloration of immature embryos of bird of paradise (Strelitzia reginae)
Authors: North, JJ 
Ndakidemi, PA 
Laubscher, Charles P 
Keywords: Oxidative browning;Activated charcoal;MS medium strength;Vitamins;Media composition
Issue Date: 2011
Publisher: Plant Omics
Source: Plant omics journal
Abstract: The optimal media composition for the in vitro germination of isolated Strelitzia reginae embryos was investigated. Different media treatments were compared to determine the effects of MS medium strength, activated charcoal (AC) and vitamin supplementation on the germination and seedling development of immature Strelitzia embryos. Results indicate the positive role of AC in reducing oxidative browning. The addition of AC (0.2 gl-¹)to the culture medium significantly reduced the discoloration of embryo explants and the culture media. Similarly, the addition of the vitamins (100 mgl-¹ Inositol, 0.1 mgl-¹ Thiamine, 0.1 mgl-¹ Pyridoxine, 2 mgl-¹ Glycine) resulted in a significant reduction in embryo discoloration. Furthermore, the addition of vitamins significantly increased root formation. Interactions between these media components resulted in significant effects. In treatments of half-strength MS with vitamin supplementation, both embryo and media discoloration were reduced. Interactions between vitamin and AC treatments presented a reduction in the embryo discoloration rate and an increased length of shoots, only when AC was absent in the media. When AC was added to vitamin supplemented media, the beneficial effects of vitamins were cancelled. The highest germination rate of embryos was observed in media containing AC without vitamin supplementation. A significant decrease in germination resulted with the addition of vitamins. The highest level of media discoloration was observed in half-strength MS media without activated charcoal and vitamin supplementation. Whilst the most effective media were; (half-strength MS without vitamins and with AC) or (half-srength MS with vitamins and without AC).
URI: http://hdl.handle.net/11189/4759
Appears in Collections:Appsc - Journal Articles (DHET subsidised)

Files in This Item:
File Description SizeFormat 
North_JJ_Ndakidemi_PA_Laubscher_CP_AppSci_2011.pdfMain article467.13 kBAdobe PDFView/Open
Show full item record

Page view(s)

27
Last Week
5
Last month
0
checked on Feb 19, 2019

Download(s)

11
checked on Feb 19, 2019

Google ScholarTM

Check


This item is licensed under a Creative Commons License Creative Commons