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Title: Optimisation of vitamin B12 and folate production by Propionibacterium freudenreichii strains in kefir
Authors: Van Wyk, Jessy 
Witthuhn, Corli R 
Britz, Trevor J 
Keywords: Vitamin B12;Propionibacterium freudenreichii (PAB);Kefir grains (KG);Poor Southern African communities
Issue Date: 2011
Publisher: Elsevier
Abstract: Vitamin B12 and folate deficiencies in poor Southern African communities lead to mandatory fortification of basic foodstuffs. Since folate- and B12-enriched kefir offers a cheaper alternative, regimes to include Propionibacterium freudenreichii (PAB) strains into kefir grains (KG) were investigated: two levels of PAB cell concentrations; freeze-drying KG to preserve PAB activity; and repeated PAB culture additions. Elevated B12 and folate levels and PCR results confirmed inclusion of PAB in all KG. Repeated inoculations with freeze-dried cultures (PAB concentration 1 × 108 cfu mL−1) delivered the highest B12 and folate production rate and concentration after 3 d. The best treatment (freeze-dried inoculum (5 × 107 PAB cfu mL−1) reacted once, followed by freeze-drying) delivered 186% Recommended Dietary Allowance (RDA) (B12) and 19% RDA (folate) per 200 mL serving. Freeze-drying preserved PAB activity in KG.
Appears in Collections:Appsc - Journal Articles (DHET subsidised)
Prof. Jessy Van Wyk

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