Please use this identifier to cite or link to this item: http://hdl.handle.net/11189/3661
Title: Aflatoxin B1-induced toxicity in HepG2 cells inhibited by carotenoids: morphology, apoptosis and DNA damage.
Authors: Reddy, L 
Odhav, B 
Bhoola, KD 
Keywords: aflatoxin B1;aflatoxin B1-N7-guanine adduct;b-carotene;DNA fragment-end labeling;HepG2;lycopene;p53 tumor suppressor gene.
Issue Date: 2006
Publisher: Walter de Gruyter
Source: Reddy, L., Odhav, B. & Bhoola, K. 2006. Aflatoxin B1-induced toxicity in HepG2 cells inhibited by carotenoids: morphology, apoptosis and DNA damage. Biological Chemistry, 387(1):87-93.
Abstract: Aflatoxin B1 (AFB1) is a fungal toxin that has been associated with primary hepatocellular carcinoma (HCC) in humans. This study was undertaken to determine the cellular and molecular mechanisms by which the antioxidants beta-carotene and lycopene inhibit AFB1-induced toxic changes in human hepatocytes (HepG2 cells). An in vitro system was optimized to test the chemoprotective effects of lycopene and beta-carotene on HepG2 cells exposed to different concentrations of AFB1. Ultrastructurally, HepG2 cells cultured in the presence of AFB1 showed mitochondrial damage, nuclear condensation and a loss of cell-to-cell contact; the latter was reflected in the observation of dysfunctional gap junctions, resulting in a loss of cell-to-cell communication. At the genomic level, AFB1 formed AFB1-N7-guanine adducts, caused apoptotic cell death and suppressed p53 protein expression. In the presence of the carotenoids, survival of cells exposed to AFB1 was increased, and there was also a significant increase in cellular mitochondrial activity. Our results demonstrate that HepG2 cells pretreated with lycopene and beta-carotene are protected from the toxic effects of AFB1 at both the cellular and molecular levels.
URI: http://hdl.handle.net/11189/3661
Appears in Collections:Dr. Lalini Reddy

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