Please use this identifier to cite or link to this item: http://hdl.handle.net/11189/3568
Title: Improving the survival of probiotic in simulated conditions and azoxymethane-induced colon tumour bearing mice using modified citrus pectin-alginate microencapsulation
Authors: Odun-Ayo, Frederick 
Mellem, John 
Reddy, L 
Keywords: modified citrus pectin;alginate;probiotic;microencapsulation
Issue Date: 2016
Publisher: African Journals Online (AJOL)
Source: Odun-Ayo, F., Mellem, J. & Reddy, L. 2016. Improving the survival of probiotic in simulated conditions and azoxymethane-induced colon tumour bearing mice using modified citrus pectin-alginate microencapsulation. African Journal of Traditional, Complementary and Alternative Medicine, 13(2):000-000.
Abstract: Background: For a probiotic to be viable it needs to be preserved at a recommended minimum level of 6–7 log10cfu/g in the product being consumed, as suggested by the International Dairy Federation. Different biopolymer matrices have been used for encapsulation of probiotic; however, loss of viability is still a challenge. Materials and Methods: Modified citrus pectin-alginate microbeads containing Lactobacillus acidophilus ATCC 4356 was developed. Efficiency of the microbeads was evaluated in simulated conditions of the gastrointestinal tract and in Balb/c mice induced with colon tumor. Genomic identification of faecal lactobacilli samples from treated mice was also performed. Results: The Modified citrus pectin-alginate probiotic microbeads significantly enhanced the viability of Lactobacillus acidophilus ATCC 4356 compared to the control (p< 0.05) both in vitro and in vivo. Exposure of the modified citrus pectin-alginate microbeads to 3 hours of simulated gastric juice resulted in 82.7% survival of L. acidophilus ATCC 4356. Also, the number of faecal lactobacilli in the modified citrus pectin-alginate probiotic treated mice increased by 10.2% after 28 days. Conclusion: Modified citrus pectin-alginate is a novel effective means of oral delivery of bacterial cells and bioactive compounds. Modified citrus pectin-alginate can be used in probiotic therapy which may improve the prevention of colon cancer.
URI: http://hdl.handle.net/11189/3568
http://dx.doi.org/10.4314/ajtcam.v13i2.13
ISSN: 0189-6016
Appears in Collections:Dr. Lalini Reddy
Appsc - Journal Articles (DHET subsidised)

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